Phage Gene Editing

Bacteriophages(phages) are a highly diverse group of bacteria-specific viruses that playirreplaceable roles in biotechnology, antibacterial therapy, microbialcommunity regulation, and basic biological research. Common model and appliedphages mainly include T-series phages (e.g., T4, T7), λ phage, M13 phage, aswell as various specific phages targeting key pathogens such as Staphylococcusaureus, Pseudomonas aeruginosa, and Escherichia coli.

Phages exhibitunique infection and lysis properties and dedicated application scenarios,offering distinct advantages across fields:

As a typicalvirulent phage, T7 phage is a widely used host for expression and display.Benefiting from its highly efficient transcription system, it occupies a coreposition in recombinant protein expression, phage display technology, and basicsynthetic biology research.

As a classictemperate phage, λ phage features dual metabolic regulatory mechanisms oflysogeny and lysis. It is widely used in the construction of genomic librariesand molecular cloning vectors, and also plays an essential role in genetransduction and studies of prokaryotic genetic mechanisms.

Specific targetphages (e.g., Staphylococcus aureus phages) serve as important toolsagainst clinical multidrug-resistant pathogens. They can act directly asantibacterial agents and are ideal carriers for delivering gene-editing toolssuch as the CRISPR system to precisely intervene in the molecular mechanisms ofbacterial drug resistance, providing key models for novel antibacterialtherapies and host-phage interaction research.

Filamentous phages(e.g., M13) show diversified application value in nanomaterials, novel vaccinedevelopment, and antibody library construction. They support high-throughputscreening of peptides and antibodies and hold promising application prospects inbiosensors and related fields.

Service Advantages

(1) HighEfficiency & Precision

Combining in vivohomologous recombination with CRISPR/Cas-based counterselection, our systemachieves high editing efficiency. Precise target design effectively avoidslethal mutations in phage genomes and reduces off-target effects.

(2) Flexibility& Versatility

We support variousediting strategies, including gene knockout, knock-in, point mutation, andlarge-fragment replacement. Customized engineering of host receptor-bindingproteins (RBPs) and loading of effector elements (e.g., CRISPR-Cas arrays) areavailable to match the life cycle and delivery requirements of differentphages.

(3) WideApplicability

Our platform coversboth temperate and virulent phages, compatible with standard laboratory modelstrains and wild-type phages isolated from environmental or clinical sources.It supports marker-free sequential editing and efficient purification and screeningof recombinant phages.

(4) ControllableTimeline